Jose Pekerman's men have lost midfielder Edwin Valencia to injury, after the Fluminense man failed to pass fit for their final squad for the Brazil showpiece.
On the brink of making Colombia's final squad is star striker Radamel Falcao, with his recovery from knee surgery clouding his availability.
Camilo Zuniga, a fullback, has also undergone the knife on his knee in October, and remains in doubt to make it to Brazil.
The fixture, though, is Pekerman's final chance to see players in a competitive situation before he is forced to name his 23-man squad for the 2014 finals on June 2.
Colombia have not tasted victory in an international since November - when they beat Belgium 2-0 - drawing with the Netherlands 0-0 and Tunisia 1-1.
Senegal have, although their opposition has not been anywhere near as strong - as they defeated Kosovo 3-1 after drawing 1-1 with Ivory Coast, Mali and Burkina Faso.
Their draw with the Ivorians saw them eliminated from World Cup qualifying, however they are now in Argentina looking to salvage some pride against the Brazil-bound Colombians.
Senegal are ranked 63rd in the FIFA standings, well below the fifth-ranked Colombians.
Akufo-Addo commissions Phase II of Kaleo solar power plant
NDC panics over Bawumia’s visit to Pope Francis
EC blasts Mahama over “false” claims on recruitment of Returning Officers
Lands Minister gives ultimatum to Future Global Resources to revamp Prestea/Bogo...
Wa Naa appeals to Akufo-Addo to audit state lands in Wa
Prof Opoku-Agyemang misunderstood Bawumia’s ‘driver mate’ analogy – Miracles Abo...
EU confident Ghana will not sign Anti-LGBTQI Bill
Suspend implementation of Planting for Food and Jobs for 2024 - Stakeholders
Tema West Municipal Assembly gets Ghana's First Female Aircraft Marshaller as ne...
Dumsor is affecting us double, release timetable – Disability Federation to ECG
