The technical team of the senior women's team have welcomed five foreign-based players into the Black Queens squad ahead of next month's Olympic qualifier.
Ghana host DR Congo in the first leg on June 3 at the Coronation Park in Sunyani.
Coach Isaac Paha and his backroom staff started preparations for the game on Saturday at the national camp in Prampram.
Four of the five foreign-based players - Mavis Danso, Patricia Ofori, Fati Mohammed and Berlinda Kanda are United States-based with Alima Moro getting a call up from his Italian base.
The 31 Queens presently in camp will be competing for places when Ghana play DR Congo at home first with the return leg scheduled for June 15.
This is the first meeting of the women's team since they rounded up their tour of the United Kingdom where the Black Queens were hosted by the FA's educational partners, the University of Hull.
Aside the 4-3 win over the women's team of Leeds United and the 14-0 thrashing of the representative side from the UK National Fire Service, the fundamental part of the Black Queens stay at the University of Hull training camp was sports science screening.
The Black Queens team had individually-prescribed physical conditioning programmes, incorporating the most contemporary training methods and hi-technology science test to assess their fitness, dietary and psychology incorporated in their training itinerary.
The programmed administered by the sports team of the University of Hull is expected to enable the Black Queens boost their preparations ahead of the Olympic qualifier and the Women's World Cup in September.
Ghana completed the double over Angola with a 4-1 aggregate result last month and continued with their steady progression for their debut at the Olympic Games.
Africa has just a slot for the 2008 Olympic Games women's football competition as eight countries are left in the qualifying campaign.
A win for Ghana over the two-legs will qualify the Black Queens for the last four which would be an all-play-all system with one side picking the sole ticket.
Aside the single slot for Africa, the runners-up from the CONMEBOL – Brazil and second-placed from the CAF qualifiers will compete in a one-off decider for a place in the Olympic competition.
Ghana is seeking to make her debut in the women's competition at the Olympic Games after narrowly missing out of the 2004 Athens Games.