Carlos Alcaraz's US Open title defence came to an end in a dramatic five-set quarter-final loss to Ben Shelton that ended at 03:34 local time - the latest finish in the tournament's history.
A large crowd remained captivated deep into the night as a modern classic unfolded over an unpredictable four hours and 28 minutes on Arthur Ashe Stadium.
The match did not begin until about 11pm in New York, after the start of the night session was delayed by Tuesday's earlier matches.
At its conclusion, eighth seed Shelton basked in a standing ovation from a delirious audience after completing a 6-7 (5-7) 6-1 6-3 1-6 7-6 (10-7) victory that will live long in the memory of all those still present.
It was the biggest - and certainly most significant - win of the 23-year-old's career.
Shelton had not beaten a top-three player in 17 attempts, and had lost each of his three previous matches against Spain's Alcaraz.
It also boosted home hopes of a first American men's singles champion in New York for 23 years.
Shelton was not born when Andy Roddick won the US Open in 2003 - but an American finalist is guaranteed when he and Frances Tiafoe meet in Friday's semi-finals.
Alcaraz was competing at his first tournament since a lengthy injury lay-off - but that is to take nothing away from Shelton, who produced a phenomenal performance.
Seven-time major winner Alcaraz had exceeded expectations on his return after more than four months out with a wrist injury, including sweeping past 20th seed Tommy Paul in straight sets to reach the last eight.
But the 23-year-old's lack of match fitness appeared to finally catch up with him in a high-octane encounter filled with breathless exchanges and astounding displays of athleticism.
Even when it appeared Alcaraz had emptied the tank, the two-time winner would not only forced a deciding set but go the distance as the players engaged in a tense 10-point match tie-break that provided a fitting conclusion to the contest.
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